non obese diabetic nod cg prkdcscid il2rgtm1wjl szj nsg mice  (Jackson Laboratory)

Journal: iScience

Article Title: Selective inhibition of ALDH1A3 impedes breast cancer growth and metastasis by blocking ALDH1A3-driven transcriptional programs

doi: 10.1016/j.isci.2026.114863

Figure Lengend Snippet: IP pharmacokinetics of CLM296 in female NOD-SCID mice (A and B) CLM296 serum concentrations versus time curve and PK parameters in female NOD-SCID mice treated with a single dose of 4 mg/kg CLM296 administered via IP injection and collected at set time points. Each point represents an individual mouse ( n = 4 per time point). (C) CLM296 organ concentrations versus time curve in female NOD-SCID mice treated with a single dose of 4 mg/kg CLM296 administered via IP injection. Each point represents an individual mouse ( n = 4 per time point). (D) CLM296 serum concentrations versus time curve in female NOD-SCID mice bearing MDA-MB-231 tumors with ALDH1A3 OE were treated with a single dose of 4 mg/kg CLM296 administered via IP injection. Each point represents an individual mouse ( n = 4 per time point). (E) CLM296 tumor concentrations versus time curve in female NOD-SCID mice bearing MDA-MB-231 tumors with ALDH1A3 OE with a single dose of 4 mg/kg CLM296 administered via IP injection. Each point represents an individual mouse ( n = 4 per time point). (F) CLM296 organ concentrations versus time curve in female NOD-SCID mice bearing MDA-MB-231 tumors with ALDH1A3 OE treated with a single dose of 4 mg/kg CLM296 administered via IP injection. Each point represents an individual mouse ( n = 4 per time point).

Article Snippet: Six to 12-week-old female non-obese diabetic severe combined immunodeficient (NOD-SCID) female mice (Charles River Laboratories, Senneville, QC) were used for all in vivo experiments.

Techniques: Drug discovery, Injection

Journal: iScience

Article Title: Selective inhibition of ALDH1A3 impedes breast cancer growth and metastasis by blocking ALDH1A3-driven transcriptional programs

doi: 10.1016/j.isci.2026.114863

Figure Lengend Snippet: Oral pharmacokinetics of CLM296 in MDA-MB-231 ALDH1A3 OE tumor-bearing female NOD-SCID mice (A and B) CLM296 serum concentrations versus time curve in female NOD-SCID mice bearing MDA-MB-231 tumors with ALDH1A3 OE were treated with a single dose of 4 mg/kg CLM296 administered via oral gavage. Each point represents an individual mouse ( n = 4 per time point). (C) CLM296 tumor concentrations versus time curve in female NOD-SCID mice bearing MDA-MB-231 tumors with ALDH1A3 OE treated with a single dose of 4 mg/kg CLM296 administered via oral gavage. Each point represents an individual mouse ( n = 4 per time point, note that at early time points, a few of the tumor samples were below the limit of detection and so are not shown in the graph). (D) CLM296 organ concentrations versus time curve in female NOD-SCID mice bearing MDA-MB-231 tumors with ALDH1A3 OE treated with a single dose of 4 mg/kg CLM296 administered via oral gavage. Each point represents an individual mouse ( n = 4 per time point).

Article Snippet: Six to 12-week-old female non-obese diabetic severe combined immunodeficient (NOD-SCID) female mice (Charles River Laboratories, Senneville, QC) were used for all in vivo experiments.

Techniques: Drug discovery

Journal: iScience

Article Title: Selective inhibition of ALDH1A3 impedes breast cancer growth and metastasis by blocking ALDH1A3-driven transcriptional programs

doi: 10.1016/j.isci.2026.114863

Figure Lengend Snippet: CLM296 reduces ALDH1A3-mediated tumor growth and metastasis of MDA-MB-231 cells in NOD-SCID mice. (A) Tumor growth and weight of MDA-MB-231 vector control tumor-bearing female NOD-SCID mice treated with 0 mg/kg ( n = 9), 0.4 mg/kg CLM296 ( n = 10), or 4 mg/kg CLM296 ( n = 10), or ALDH1A3 OE tumor-bearing mice treated with 0 mg/kg ( n = 9), 0.4 mg/kg ( n = 10), or 4 mg/kg ( n = 9) CLM296. Treatment with CLM296 began once palpable tumors developed (indicated by the arrow, day 15) in the tumor volume plot, which demonstrates weekly caliper measurements. The tumor weight plot shows final tumor weight from the harvested tumors at endpoint. Tumor volume and weight significance were analyzed with two-way ANOVA with multiple comparisons post-test and p value < 0.05 = ∗, <0.01 = ∗∗, <0.001 = ∗∗∗, <0.0001 = ∗∗∗∗, ns, not significant. In the tumor volume graph, each point represents the mean of each group and error bars represent standard error of the mean (SEM). In the tumor weight graph, each point represents an individual mouse, and error bars represent SEM. (B) RNA extracted from the harvested tumors was analyzed via RT-qPCR for DHRS3 and RARβ expression. Significance was analyzed with two-way ANOVA with multiple comparisons post-test and p value < 0.05 = ∗, <0.01 = ∗∗, <0.001 = ∗∗∗, <0.0001 = ∗∗∗∗, ns, not significant. Each point represents an individual mouse and error bars represent SEM. (C) Body weights of the mice were measured weekly. Arrow indicates start of CLM296 IP injections. Each point represents the mean of each group and error bars represent SEM. (D) Serum creatinine and ALT of MDA-MB-231 vector control or ALDH1A3 OE tumor-bearing mice when treated daily with CLM296 via IP injection for 26 days. Significance was analyzed with two-way ANOVA with multiple comparisons post-test and p value < 0.05 = ∗, <0.01 = ∗∗, <0.001 = ∗∗∗, <0.0001 = ∗∗∗∗, ns, not significant. Each point represents an individual mouse and error bars represent SEM. (E) Transwell invasion assays were completed with vector control and ALDH1A3 OE MDA-MB-231 cells treated with or without 100 nM CLM296. FBS was used as a chemoattractant. Significance was determined by one-way ANOVA followed by Tukey’s multiple comparisons test and p value < 0.05 = ∗, <0.01 = ∗∗, <0.001 = ∗∗∗, <0.0001 = ∗∗∗∗, ns, not significant. Each point represents a separate n ( n = 6), and error bars represent SD. (F) Representative images of H&E-stained mouse lung sections from the second in vivo experiment taken from mice bearing MDA-MB-231 ALDH1A3 OE tumors treated with or without 4 mg/kg CLM296 ( n = 5 per group; 1 independent experiment). Arrows indicate metastatic lesions. (G) Quantification of MDA-MB-231 ALDH1A3 OE cells in the lung lobes of each mouse by RT-qPCR using human specific GAPDH primers. The horizontal line represents the limit of detection in the assay at 10 MDA-MB-231 per mouse lung lobe. Significance was analyzed with a one-way unpaired t test, and p value < 0.05 = ∗. Each point represents an individual mouse ( n = 9), and error bars represent SEM.

Article Snippet: Six to 12-week-old female non-obese diabetic severe combined immunodeficient (NOD-SCID) female mice (Charles River Laboratories, Senneville, QC) were used for all in vivo experiments.

Techniques: Plasmid Preparation, Control, Quantitative RT-PCR, Expressing, Injection, Staining, In Vivo

Journal: Microbiology Spectrum

Article Title: A presumed mouse parvovirus with overlooked high toxicity for human primitive CD34 + hematopoietic precursors in vitro and in bone marrow-humanized mice

doi: 10.1128/spectrum.02339-25

Figure Lengend Snippet: Hematopoietic parameters in MVM-infected BM-humanized immunodeficient mice. ( A ) General scheme of NSG mice handling. The timeline of human CD34+ cell transplantation in sub-lethally irradiated mice, viral strain infection, and sampling of hematopoietic organs is outlined. ( B ) Impact of the MVM strains on human hematopoiesis in BM-humanized mice. Shown are percentages of total CD45+ human hematopoietic cells (upper), or the subpopulation of primitive CD34+ precursors (lower) monitored by flow cytometry in control mock, MVMp-, and MVMi-infected BM-humanized NSG mice. Numbers in parentheses indicate the factor of human precursor decay by MVMi or MVMp infection compared to those in mock mice. * P value < 0.05; ** P value < 0.01. ( C ) A representative example of the differentially unbalanced mouse/human hematopoiesis in MVMp- and MVMi-infected mice at 120 dpi. Left: total mouse (mCD45) versus human (hCD45) hematopoietic cells; right: primitive human CD34+ precursors versus mouse total (mCD45) hematopoietic cells. Numbers within the window indicate the percentage of positive cells stained by the respective antibodies.

Article Snippet: Non-obese diabetic immunodeficient NOD.Cg- Prkdc scid Il2rg tm1Wjl /ThomJ (NSG) mice (purchased from The Jackson Laboratory) were used.

Techniques: Infection, Transplantation Assay, Irradiation, Sampling, Flow Cytometry, Control, Staining

Journal: Microbiology Spectrum

Article Title: A presumed mouse parvovirus with overlooked high toxicity for human primitive CD34 + hematopoietic precursors in vitro and in bone marrow-humanized mice

doi: 10.1128/spectrum.02339-25

Figure Lengend Snippet: MVM strain replication, pathogenicity, and evolution in normal and BM-humanized immunodeficient mice. ( A ) Quantitation of MVM genomes in mice. Viral genomes quantitated by qPCR with MVM-specific primers in low-molecular DNA isolated from the indicated organs from basal (left) and bone marrow-humanized (right) NGS mice. Shown are data obtained from BMp, BM, and spleen (S) from MVMp- and MVMi-infected mice. Samplings were performed at the post-infection times outlined in . The dotted line marks the reliable detection limit of the qPCR. Statistical significance: P = 0.0332 and P = 0.0107, respectively (* P < 0.1). ( B ) Kaplan-Meier representation of NSG and h/NSG mouse survival subjected to the indicated treatments along 150 days post-infection. ( C ) Localization of the amino acid changes in the MVM capsid arising along the evolution of both viral strains in basal and BM-humanized NGS mice. The 3-D crystal structure of the MVMp and MVMi capsids is illustrated using Visual Molecular Dynamics and the PDB coordinates 1Z14 and 1Z1C, respectively. Amino acid changes are highlighted by arbitrary colors indicated for each type of residue.

Article Snippet: Non-obese diabetic immunodeficient NOD.Cg- Prkdc scid Il2rg tm1Wjl /ThomJ (NSG) mice (purchased from The Jackson Laboratory) were used.

Techniques: Quantitation Assay, Isolation, Infection, Residue